Development of an Electrochemical DNA Biosensor for Vibrio parahaemolyticus

Vibrio parahaemolyticus has been considered as one of the most important food-borne bacterial pathogens. Because of the safety concerns, detection and characterization of V. parahaemolyticus have attracted much attention. Recently many researchers are focusing towards the progress of rapid methods. Therefore the development of biosensors is an alternative way to improve the sensitivity, selectivity, rapidity, reliability, effectiveness and suitable for in situ analysis. From previous research, it has been proved that electrochemical DNA biosensors exhibit the advantages such as high sensitivity, selectivity, simplicity, low cost, rapidity of response and ease of miniaturization. The approach of this research will be demonstrated by employing the DNA probe to a modified electrochemical cell which formed the basis of a DNA biosensor. The electric signals generated from the DNA biosensor will be imposed using voltammetry technique (CV and DPV). The effect of hybridization and non-specific binding will be examined. The performance of the DNA biosensor will be optimized and validated using seafood sample. The specific binding of V. parahaemolyticus DNA probe to its complementary DNA will be employed in this study for targeting specific identification of V. parahaemolyticus. The capabilities of electrochemical transducer systems with screen-printed electrode will be performed in this work.

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Prepared by: Noordiana Nordin (GS32185)