Lateral Flow Immunoassay for ultrasensitive and affordable naked eye detection of Tuberculosis

In this study, Lateral Flow Immunoassay was used in detection of active tuberculosis (TB). Tuberculosis (TB) is still one of the greatest health care problems in the world. The development of a rapid and accurate test for the diagnosis of TB is a priority for TB control. Traditional diagnostic techniques based on the isolation of the tuberculosis bacillus in culture media are time consuming, and it is necessary to wait for several weeks to obtain a result. In this industrialized world many complex and expensive techniques are available, but these techniques cannot be used because of their high cost and lack of trained personnel. Therefore, a possible biosensors that easy to use and cheap is the lateral flow immunoassay (LFIA), which is affordable, sensitive, specific, user-friendly and so on. LFIA is based on recognition of one or more analyte, mainly proteins, by using antibodies. The antibodies are fixed onto a nitrocellulose membrane and they interact with the analyte either in sandwich or competitive formats using a proper label.

Figure 1: Lateral Flow Architecture
 

Prepared by: Nazifah Ariffin (GS32185)