Immuno Nanosensor for Ultrasensitive and Affordable Naked Eye Detection of Tuberculosis
Airbone infectious disease known as tuberculosis is a global emergency which caused by Mycobacterium tuberculosis. Failure to control the spreading of Mycobacterium tuberculosis transmissions allowing continued infection-control challenge. An attractive approach for ultrasensitive and affordable naked eye for diagnosis tuberculosis utilizing plasmonic enzyme-linked immunosorbent assay (ELISA) for detection of tuberculosis via antibody-antigen interaction has been studied for tuberculosis. In this technique, the biocatalytic reaction of catalase consuming hydrogen peroxide which involved in reducing the gold ion solution to produce blue and red color solutions in the presence and absence of specific antigen; M. tuberculosis ESAT-6-like protein EsxB2 respectively. The plasmonic ELISA offers advantage since conventional ELISA only shows tonality colors that need high concentration of analyte to achieve high confident level for naked eye detection. The developed immune nanosensor provides rapid, highly sensitive, easy to perform and user friendly that could be implemented in conditions found in developing countries.