DNA and immuno based biosensors system for ultrasensitive, non-invasive and affordable detection of mycobacterium tuberculosis (TB)

A novel method for rapid detection of mycobacterium tuberculosis in urine

Summary

In Resource-constrained countries, affordable methodologies for the detection of disease biomarkers at ultralow concentrations can potentially improve the standard of living. However, current strategies for ultrasensitive detection often require sophisticated instruments that may not be available in laboratories with fewer resources. Here we propose a signal generation mechanism for biosensing that enables the detection of a few molecules of analyte with naked eye detection. The enzyme label of an enzyme-linked immunosorbent assay (ELISA) controls the growth of gold nanoparticles and generates coloured solutions with distinct tonality when the analyte is present.

Electrochemical transduction integrated with nano-based material has attracted much attention because of several important properties. The main advantage of this system is that it is an extremely sensitive sensor, capable of detecting subnanogram levels in real time without any specific label [11]. Moreover, electrochemical biosensor can detect trace amounts of specific analytes from complex fluids without or less sample preparation. Due to these advantages, electrochemical biosensor has emerged as a powerful tool that can greatly provide valuable information on biomedical and chemical analyses. Therefore in this study we proposed nanomaterial based electrochemical PNA biosensor for the determination of M. tuberculosis by using simple non-invasive technique (salivary and urinary sample). Nanomaterial used in this work will be nanographene and quantum dots. PNA will be used as probes for hybridization detection with complementary target of M. tuberculosis of genomic DNA in clinical samples.