Multifunctional nanofluidic biochip for dengue detection utilizing silicon nanostructure

A novel method for rapid detection dengue

Summary

There is an urgent need to develop rapid, simple, cost-effective medical devices for screening medical diseases for example dengue for early medical diagnosis. Such a system will also be useful in physician offices or for personal use at home. Initial Dengue virus infection symptoms are very similar to those of influenza, measles, malaria, typhus, yellow fever, and other virus infections, which make the diagnosis based on presenting symptoms problematic. ELISA assays for the detection of IgG and IgM antibodies to Dengue virus are available but unfortunately they are compromised by cross-reactivity with other flaviviruses and require at least 5 days post infection to mount a sufficient immune response to produce detectable antibodies in a patient. This might be too late for patients with severe dengue infection as complication e.g. bleeding tendency and plasma leakage occur at the same time.

Thus we are proposing the development of a molecular assay method based on DNA for the detection of dengue virus. This will be combined with lateral flow technology (nanofluidic) incorporating the use of nanomaterial for signal amplification to develop a point of care (POC) biosensor which will enable the early and highly accurate detection of the virus. The product will also have the added advantage of short operation time and labor-free processing.

Figure 1:
(a) the top view of 60nm width SiNW
(b) FESEM image of the fabricated nanowire aligned with Au pad