An Electrochemical DNA biosensor based on SiNWs/AuNPs-modified electrode for early dengue virus detection
Dengue virus diseases arisen as one of the serious public health problems for the world population. Worst, there is no specific medication or antibiotic to kill the dengue virus, but just solely depend on the treatment to relief from the dengue virus infection symptoms. Therefore, the development of rapid and cheaper diagnostic detection of dengue virus at an early stage is seemed as an important effort today. The electrochemical detection based on DNA dengue virus hybridization, can offer an advantageous for fast sensing and direct due to can convert the hybridization event into a direct electrical signal. As the strategy to enhance the electrochemical responses of DNA dengue detection, electrode modification utilized of combined SiNWs with AuNPs was investigated. This work describes the incorporation of SiNWs/AuNPs composite as sensing material for DNA electrochemical detection. SiNWs/AuNPs composite was further explored as matrix for DNA probe immobilization where the DNA dengue virus, synthetic was used as bio-sensing model to evaluate the performances of the DNA electrochemical sensor. The hybridization of complementary DNA was monitored by differential pulse Voltammetry (DPV) using methylene blue (MB) as redoxindicator. The fabricated biosensor has been shown to be highly specific when evaluated using real sample of the genomic DNA dengue virus (PCR amplicons) of DENV type 1- spikedserum, DENV type 2-spiked serum, negative control and DENV- cell culture and also demonstrated the detection range of 360 ng/uL-5 ng/uL.
Fabrication of DNA Electrochemical Sensor based on SiNWs/ANPs modified electrode